FastQCFastQC Report
Sun 14 Apr 2019
SRR6576009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360600
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29830.21924151109804496No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22340.1641922681170072No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20890.15353520505659268No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18830.13839482581214171No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15090.1109069528149346No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15020.11039247390856974No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14950.10987799500220491No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14670.10782007937674555No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13780.10127884756724974No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13670.10047038071439071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20650.059.5110471
GGTATCA7050.052.7612571
TCAACGC27600.043.0782284
ATCAACG27850.043.0290153
CAACGCA29050.040.9280245
AACGCAG30250.039.3044326
ACCTAAG9800.035.5535351
TATCAAC35200.034.580832
TAGGTAT7150.033.5205045
GTCTTAG9650.031.714891
AGGTATA7150.031.548716
TTAGGTA7500.031.329624
ACGCAGA38450.031.0444377
CTAAGAC13100.030.8513073
CTTAGGT7400.030.4828763
CGCAGAG39650.030.1237038
TAAGACA13600.029.3715214
GTACATG54300.029.0483971
CCTAAGA13300.028.976142
GGTATAG7700.028.6849147