FastQCFastQC Report
Sun 14 Apr 2019
SRR6576010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408223
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18780.133359560240104No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18400.13066112398391447No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16300.11574871309444597No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16060.114044437564221No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15820.11234016203399604No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15810.11226915055357No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15150.10758239284545132No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15120.10736935840417319No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14980.10637519767820863No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14920.1059491287956524No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14660.10410283030457534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23900.057.2419621
GGTATCA9000.052.2368431
ATCAACG30950.044.340733
TCAACGC31400.043.7052734
CAACGCA33050.042.092135
AACGCAG34150.041.562046
ACGCAGA40750.034.830527
TATCAAC40150.034.776862
CGCAGAG44050.032.1145028
GTACATG55100.029.5218751
GCAGAGT48950.029.0917979
TACATGG55950.028.5692142
ACATGGG58650.027.0048963
AGAGTAC41800.025.8602210-11
ACTACGC1101.0187707E-425.6353473
GTACTAG950.001302604424.7437671
TAGGTAT5900.023.8973585
GTACTAT1602.0343887E-623.506581
CTAAGAC11500.023.294733
TAAGACA11600.023.0939124
Produced by FastQC (version 0.11.2)