FastQCFastQC Report
Sun 14 Apr 2019
SRR6576011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245601
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17810.14298318642968336No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17780.14274233883884163No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15070.12098577313280898No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14900.1196209701180394No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14510.11648995143709742No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14340.11512514842232785No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14120.11335893275615547No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13450.10798000322735772No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13100.10517011466753799No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12830.1030024863499628No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12780.10260107369855996No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12690.1018785309260349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.062.2126121
GTATCAA20700.059.9389271
TCAACGC27300.044.4063724
ATCAACG27500.044.0834163
CAACGCA28750.042.165065
AACGCAG29450.041.3223766
TATCAAC34600.035.0444342
ACGCAGA37850.032.1517567
CTTAGGT5850.030.5222073
CGCAGAG40850.029.7905488
GTCTTAG7900.029.7452961
GTACATG51550.029.0828821
TTAGGTA6100.028.5010014
AGGTATA6100.028.4998576
ATAGTAC1503.7074642E-828.1928833
TAGGTAT6400.027.8980885
TACATGG53500.027.5836222
GCAGAGT44900.027.2080769
GGTATAG6400.027.1639297
ACATGGG55300.026.4255183
Produced by FastQC (version 0.11.2)