FastQCFastQC Report
Sun 14 Apr 2019
SRR6576012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948872
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC21990.23174885548314209No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17370.18305946429023093No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15010.15818782723064861No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13790.14533045553035603No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13270.1398502643138379No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13180.13890176968020976No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12900.1359508974867No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12810.13500240285307186No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12240.12899527017342696No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12070.12720366919879605No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11590.12214503115277929No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11530.1215127013970272No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11340.11951032383714558No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10830.11413552091325278No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10800.11381935603537675No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10070.10612601067372628No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9510.10022426628670675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23900.052.715931
CAACGCA28450.042.291035
ATCAACG28650.041.9958043
AACGCAG33350.037.204776
TCAACGC33600.035.808924
GGTATCA10600.035.0369451
ACGCAGA34800.034.4390757
CGCAGAG36050.033.3753058
TATCAAC39700.032.564712
GCAGAGT38500.031.2514259
TTAAGGT1701.2187229E-1030.4112534
CATGGGT8050.028.6082154
TAGGCAT5950.028.4364955
TAGTACT8550.027.4849194
GACGTTA1205.8425358E-627.4162067
GTACTAG4650.027.2970541
AACGGCG350.00883816926.85669132-33
CTAGGCA6200.026.5318134
AGAGTAC35000.026.52098310-11
ACATGGG39700.026.1632863
Produced by FastQC (version 0.11.2)