FastQCFastQC Report
Sun 14 Apr 2019
SRR6576013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883928
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20220.22875166303137812No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17570.1987718456706881No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15400.17422233485080232No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12380.1400566561982424No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12320.13937786788064185No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12130.13722837154157352No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12060.13643645183770625No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11780.1332687730222371No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11440.12942230588916745No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11330.12817786064023315No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11280.12761220370889936No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10620.12014553221529356No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10270.11618593369595714No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC10180.11516775121955633No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10160.11494148844702283No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC9920.11222633517662071No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9760.11041623299635264No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9550.1080404738847508No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9500.10747481695341703No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA9220.10430713813794788No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT8850.10012127684607797No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG8840.10000814545981121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.051.9521561
GGTATCA9650.039.9457631
CAACGCA28150.039.560115
ATCAACG28900.038.5356453
GACGTTA1551.8189894E-1236.3778577
GTACTAG5300.036.3657151
AACGCAG32350.036.1670076
CTAACTG2400.035.2430424
TCAACGC33350.033.675514
ACGCAGA33350.033.2509237
TATCAAC36150.032.3817062
ACGTTAA1755.456968E-1232.2203878
CGCAGAG34600.032.1854638
CATGGGT7050.031.3271484
TCAAGAC2600.030.7247053
GCAGAGT37000.030.2230439
TATTCTC3450.029.9634065
TAGGCAT6900.029.9634065
CGTTAAC1901.4551915E-1129.6749949
CTAGGCA7650.029.4843754
Produced by FastQC (version 0.11.2)