FastQCFastQC Report
Sun 14 Apr 2019
SRR6576014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384868
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.15303948366712744No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5800.1507010195703462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10950.046.0156631
ATCAACG14500.035.0061723
TCAACGC14750.034.4128464
AACGCAG14700.034.2101756
CAACGCA14850.034.181115
GTGTTAC1052.0298394E-631.3938641
TATCAAC16400.030.9505792
GTACATG22950.030.77831
CATGGGG8900.030.6285784
ACGCAGA16500.030.4781557
TACATGG23450.030.2637632
ACATGGG24100.029.642543
CGCAGAG17000.029.581748
GGTATCA5550.027.1514511
GGGTACC2950.027.0841877
GCAGAGT19050.026.3984039
CATGGGT4750.025.725784
GGTACCT2850.024.736338
ATGGGAG5950.024.4868875
GAGTACA15300.024.3148561
Produced by FastQC (version 0.11.2)