FastQCFastQC Report
Sun 14 Apr 2019
SRR6576015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886112
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11710.1321503376548337No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11580.13068325448701743No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10530.11883373659311691No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10510.11860803149037592No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9200.10382434726084286No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9090.10258296919576759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16000.060.527871
GGTATCA4500.055.3696141
TCAACGC22600.042.007664
ATCAACG22750.041.730693
CAACGCA23800.039.8896335
AACGCAG24750.038.168626
TATCAAC26850.036.068752
ACGCAGA29750.031.7537257
GTACATG38500.029.5503621
CGCAGAG32700.028.8890938
TACATGG39300.028.8292032
ACATGGG39400.028.2707773
GCAGAGT34000.027.922749
AGAGTAC30400.025.35454810-11
ATGGGAG10050.025.253025
TAAGACA6150.025.21884
ATTATCC6700.024.5515463
CTTAGGT2900.024.309663
CATGGGA17750.023.8303154
GTCTTAG4350.023.7761521
Produced by FastQC (version 0.11.2)