FastQCFastQC Report
Sun 14 Apr 2019
SRR6576015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886112
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11920.1345202412336138No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11810.13327886316853851No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11310.12763623560001444No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9580.10811274421292116No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9300.10495287277454768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.064.330711
GTATCAA16900.062.4488871
TCAACGC23700.044.4255184
ATCAACG24100.043.4931223
CAACGCA24150.043.0138135
AACGCAG24650.042.522696
TATCAAC28300.036.706132
ACGCAGA30000.034.465557
CGCAGAG32850.031.4753888
GCAGAGT34050.030.2280969
GTACATG38400.029.2017651
CTAAGAC6600.029.1993183
TACATGG39250.028.6214122
TAAGACA7000.028.202274
TAGGTAT2350.028.0022535
ACATGGG40100.027.6630743
ACCTAAG4650.026.3441261
AGAGTAC30500.025.88768610-11
GTACTAG1101.0012407E-425.6993391
GTCTTAG4100.025.2814641
Produced by FastQC (version 0.11.2)