FastQCFastQC Report
Sun 14 Apr 2019
SRR6576017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963306
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26010.13248062197130758No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23720.120816622574372No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22470.11444981067648141No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22250.11332925178245266No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA22060.1123614963739733No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22030.1122086928884239No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21170.10782832630267518No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20890.10640216043754769No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20540.10461945310613831No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20520.10451758411577207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29650.062.3047941
GGTATCA10200.048.849411
ATCAACG41750.043.790553
CAACGCA42300.043.6656045
TCAACGC41900.043.633784
AACGCAG44450.041.7650156
TATCAAC50350.036.9699942
ACGCAGA54500.033.9771547
GTACATG80250.032.39171
TACATGG80800.032.054862
CGCAGAG60050.030.8368848
ACATGGG84400.030.5158443
ACCTAAG9450.029.8451041
TAGGTAT7050.027.9993175
GCAGAGT66750.027.6712369
TTAGGTA6950.027.0497024
AGAGTAC54900.025.76804410-11
GTCTTAG10250.025.6813451
AGGTATA6600.025.6357386
TAAGACA13950.025.6051124