FastQCFastQC Report
Sun 14 Apr 2019
SRR6576021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243218
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16460.13239834043586887No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14430.11606974802488382No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14380.11566756594579551No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13590.10931308909620036No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12950.10416515848387009No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12630.10159119317770496No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12550.10094770185116368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.059.7731061
GGTATCA6500.057.9988021
ATCAACG25400.045.3372653
TCAACGC25600.044.799464
CAACGCA26300.043.964525
AACGCAG27900.041.4432566
ACCGTAT500.001702606637.5976338
TATCAAC32600.034.8915862
TTAACGC550.002716323634.1837963
GTCTTAG5400.034.0340231
ACGCAGA35450.032.61297
CGCAGAG36800.031.2887948
GTACATG48400.031.1563821
TACATGG48750.030.8530642
ACATGGG49900.030.3304083
AGGTATA3050.029.2803786
CTTAGGT3450.028.6103483
ATAGTAC1652.9631337E-928.4864943
GCAGAGT40250.028.2566099
TAGGTAT3200.026.4390265
Produced by FastQC (version 0.11.2)