FastQCFastQC Report
Sun 14 Apr 2019
SRR6576023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131587
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13430.11868287634976364No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12550.1109061875048052No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11570.10224578401837418No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11470.10136206937690165No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11340.10021324034298733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.056.8316461
GTATCAA17900.056.33331
ATCAACG24450.039.792443
TCAACGC24800.039.2308544
CAACGCA25350.038.1942835
AACGCAG26950.036.7987256
TATCAAC30400.032.0041162
GTACATG46850.030.5751381
ACGCAGA33250.029.963727
TACATGG48050.029.6386432
ACATGGG48050.029.2473753
CGCAGAG35800.027.698168
GTCTTAG5400.027.050321
ACCTAAG4850.025.2601591
TTAGGTA3350.025.2543854
TAGGTAT3350.025.2543855
CTTAGGT3550.025.155583
GCAGAGT40150.024.6972379
ATTATAC2501.6370905E-1124.4406343
AGGTATA3350.023.8513646
Produced by FastQC (version 0.11.2)