FastQCFastQC Report
Sun 14 Apr 2019
SRR6576024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971088
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14060.1447860544049561No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11200.11533455258431778No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10800.11121546142059216No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10690.11008271135056762No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10530.10843507488507735No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9970.10266834725586146No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9950.10246239269767518No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9870.10163857446493006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.057.4598731
GGTATCA5000.051.699151
TCAACGC21100.041.4220664
ATCAACG21300.041.0331233
CAACGCA21800.040.0919955
AACGCAG22400.039.4376536
GTCTTAG5350.034.2611161
TATCAAC26250.033.6621322
GTACATG38700.032.5472681
TAGGTAT3850.031.73325
ACGCAGA28050.031.4938857
TACATGG39550.031.3740462
TTAGGTA4200.030.2075654
CGCAGAG29450.029.9967218
ACATGGG40900.029.9859943
ACCTAAG5800.029.9822641
AGGTATA4000.028.1937276
CTTAGGT4350.028.0857073
CTAAGAC7750.027.2842543
GCAGAGT32900.026.7069689