FastQCFastQC Report
Sun 14 Apr 2019
SRR6576024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971088
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12290.12655907600547014No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12110.12470548498179361No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10540.1085380521641705No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10370.1067874384195871No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9840.10132964262765064No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9780.10071177895309179No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9770.10060880167399863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16600.058.9491841
GGTATCA5900.054.222491
TCAACGC20600.047.2208064
ATCAACG21150.045.9928473
CAACGCA21400.045.016365
AACGCAG22200.043.8175056
TATCAAC25800.038.249862
GTACTAG906.141745E-736.591321
ACGCAGA28100.034.4501537
CGCAGAG28550.033.7669038
TGCCGTA1309.198629E-932.53342
GTACATG39200.031.5640141
TACATGG40200.030.5101782
ACATGGG40150.029.962963
GCAGAGT31750.029.7711649
TAGGTAT2950.027.0805325
ACCTAAG5700.026.411781
CATATAC2500.026.3159053
CCCTATA1101.0192542E-425.6323762
CTTAGGT3350.025.2498043
Produced by FastQC (version 0.11.2)