FastQCFastQC Report
Sun 14 Apr 2019
SRR6576025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345297
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5640.16333764845915255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.11062940019751114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10250.045.3967321
CTATAGC550.002717773734.173133
AACGCAG14450.032.842866
ATCAACG14300.032.530193
TCAACGC14450.032.5176854
CAACGCA14650.032.0737575
GTACATG21550.032.061461
TACATGG21300.031.5551072
CATGGGG9000.031.3253674
ACATGGG20800.031.1747653
TATCAAC15150.030.4037192
ATGGGGA4850.030.0336025
ACGCAGA16350.029.026267
ACTATAC1154.1935127E-628.6014233
CGCAGAG16750.028.3330968
TACTATA1904.4383341E-1027.2114932
CGCGGTA1807.479684E-926.10447394
GCAGAGT18600.025.5150179
ATGGGAG4850.023.2518225
AGAGTAC17700.022.96308710-11
Produced by FastQC (version 0.11.2)