FastQCFastQC Report
Sun 14 Apr 2019
SRR6576025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345297
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5740.16623370605594606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.16275843693979386No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3540.10252043892648938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.043.2069471
GTACATG22250.034.289811
GGTACCT2800.033.5860758
ATGGGTA3400.033.1717155
TACATGG23000.033.0996062
CATGGGG7950.032.5110634
ACATGGG23350.032.4022063
CATGGGT5150.031.937174
AACGCAG14800.031.1171636
TGGGTAC3200.030.839336
GGGTACC3050.030.8152547
ATCAACG15150.030.3982833
TCAACGC15250.030.1989524
TCCGACC953.733625E-529.6971598
CAACGCA15550.029.6163355
ATGGGGA4900.028.7713855
TATCAAC17300.027.7070122
GTATTAT856.7259866E-427.7032781
GTATTAC1205.736316E-627.4724161
CCTAGAC1057.41403E-526.8532923
Produced by FastQC (version 0.11.2)