FastQCFastQC Report
Sun 14 Apr 2019
SRR6576027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366467
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16160.11826118010899642No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14430.10560079387208034No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14370.10516170533207168No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14100.10318580690203275No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14080.1030394440553632No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13670.10003900569863743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.058.926751
GGTATCA7450.054.308551
ATCAACG28000.040.447543
TCAACGC28150.040.2320144
CAACGCA29700.037.9741365
AACGCAG30550.037.379046
TATCAAC35800.031.8998152
GTACATG55050.030.5096281
ACGCAGA37950.030.0903747
ACATGGG54600.029.865513
GTATACC953.7276237E-529.713491
TACATGG56450.029.5548882
CGCAGAG39500.028.9297268
GCAGAGT44850.025.7933549
GTCTTAG6700.025.278641
CTAAGAC8200.024.642673
AGAGTAC37250.024.36498610-11
CATGGGG31850.023.6071554
CATGGGA26000.023.1350124
ATGGGAG15750.022.9743525
Produced by FastQC (version 0.11.2)