FastQCFastQC Report
Sun 14 Apr 2019
SRR6576030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726571
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20570.2831106664042468No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17060.23480155414956008No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14770.20328364330533424No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14340.197365432972139No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12000.16515935813568117No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10880.14974448470968427No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10050.13832096243863298No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9820.13515540807436574No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9800.13488014247747293No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT9550.13143932251631293No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9310.1281361353535993No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9240.12717270576447448No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC8890.12235555781885045No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8110.11162019954003119No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT8060.1109320355477992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.10969334036178158No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC7920.10900517636954957No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT7760.10680305159440714No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7750.10666541879596075No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7610.10473855961771114No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7350.10116010685810471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA407.90024E-658.912871
GTATCAA19300.054.700451
GGTATCA8350.040.0748631
CAACGCA25650.039.579415
ATCAACG25950.039.124543
GTACTAG5100.038.8131871
AACGCAG30450.035.0381776
TAGGCAT6250.034.5924075
TATCAAC31050.034.514862
GCGTACA550.002679727734.276581
ACGCAGA30350.033.602697
CGCAGAG30900.033.1566858
TCAACGC31250.032.4867824
GCAGAGT32400.031.9117589
TATACTG1351.3256795E-831.3337025
CTTGCGT600.004161146531.3315458
AACCGTG1807.2759576E-1231.3315437
CTAGGCA6950.031.1082764
GAACCGT1859.094947E-1230.4868436
ACGTTAA1853.274181E-1027.944358
Produced by FastQC (version 0.11.2)