FastQCFastQC Report
Sun 14 Apr 2019
SRR6576036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424222
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.16076488253791646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.034.902521
CCGACCG600.004158917331.33199994
GTACATG20400.028.4443151
CAACGCA15050.026.8559975
ACTAATG700.0088316926.8559953
TACATGG21050.026.7985252
ATCAACG15150.026.6787323
ACATGGG21150.026.4433173
TCAACGC15600.026.2104194
CATGGGG9850.025.76544
AACGCAG16100.025.6883476
TATCAAC16700.024.2082672
GTCCTAT1000.001719064723.5879671
ACGCAGA17850.022.9065887
CGCAGAG18050.022.6527738
TAGGCGG1050.002333663622.3799975
AATAGAC1050.002333663622.3799973
AGTAAGG1100.00305297621.3677642
GCGAGCT1100.00305715521.3627269
CATGGGT4750.020.778064
Produced by FastQC (version 0.11.2)