FastQCFastQC Report
Sun 14 Apr 2019
SRR6576042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001586
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC29890.29842669526131554No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT23380.2334297803683358No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC21250.21216350867524106No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT18180.18151212177486506No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA15590.15565313412927098No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG14680.1465675438754136No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC14150.14127593636492522No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA13730.13708258701699105No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13720.13698274536584976No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13550.13528543729644785No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13180.1315912962042201No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12370.12350412246177564No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT11940.11921093146270015No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT11400.1138194823010705No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11100.1108242327668318No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10830.10812850818601699No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA10560.10543278360520215No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT10410.1039351588380828No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10320.1030365839778112No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10190.10173864251297443No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10100.10084006765270281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25650.050.0367051
ATCAACG31900.039.1899153
CAACGCA32100.038.945745
GTACTAG6250.038.362211
AACGCAG38500.034.424826
ACGCAGA37600.033.248897
CGCAGAG38300.032.6412058
CTGTGCG10250.032.5550089
TATCAAC39500.032.492192
GGTATCA11800.031.8728891
GCAGAGT39450.031.6896889
GTATATA1501.0659278E-931.3416731
TAGTACT10050.031.3322874
TCAACGC40400.030.944514
GTACTGT12000.028.590716
CTAGTAC11950.028.3170433
TAGGCAT8650.028.2533915
TGGGTAC4700.027.9990656
CTAGGCA9100.027.8891754
TAACCAC2203.6379788E-1227.7717976
Produced by FastQC (version 0.11.2)