FastQCFastQC Report
Sun 14 Apr 2019
SRR6576043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903803
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC25910.2866775171137958No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT19250.21298889249095213No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC16180.17902131327291454No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT15010.16607601435268526No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA14070.1556755177842959No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12810.14173442663943359No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA11930.13199779155413294No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11420.12635496894787912No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10980.1214866514052288No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT10820.11971635411699232No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT10720.1186099183118445No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10520.1163970467015489No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10490.11606511596000456No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10430.11540125447691589No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10250.11340967002764983No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9880.10931585754860296No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9420.10422625284492307No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9350.10345174778131962No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC9350.10345174778131962No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9310.10300917345926047No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT9130.10101758900999444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21550.048.4802741
CAACGCA27300.037.6977735
GTACTAA500.001691060737.6486431
ATCAACG27000.037.594493
ACCTAAG1750.034.9594541
GTACTAG6150.034.434741
AACGCAG32500.034.124236
ACGCAGA31350.032.8277247
CGCAGAG32200.032.1302038
GCAGAGT33750.030.5152559
TATCAAC34700.030.2018572
TAGGCAT8000.029.9581095
TCAACGC34650.029.8368954
AAGCTAA3800.029.7012219
GGTATCA10100.029.354761
GTACATG36650.029.1481671
TACATGG37000.028.8325042
ACATGGG36400.028.7897363
GTATAAG6750.028.5850811
TAACCAC2002.7284841E-1128.1958665
Produced by FastQC (version 0.11.2)