FastQCFastQC Report
Sun 14 Apr 2019
SRR6576044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993119
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15660.1576850306962207No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15370.1547649375351796No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13500.13593537128984542No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12710.1279806347476989No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12330.12415430577805882No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12080.12163698408750613No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11870.11952243386744188No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10940.11015799717858585No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10240.1031094964450383No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10150.10220326063643934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17050.058.183761
GGTATCA5600.054.5719031
TCAACGC23850.041.1858984
ATCAACG24150.040.868893
CAACGCA24250.040.5065425
AACGCAG24600.040.1212846
TATCAAC28750.034.832572
ACGCAGA29200.033.8008087
GTACATG40250.031.5385441
ACATGGG40300.031.0218163
TACATGG40850.030.8451212
CGCAGAG31950.030.7444028
GTCTTAG5450.029.3309521
GCAGAGT33700.029.1478849
TAGGTAT4000.028.1995335
ACCTAAG6400.027.1810041
CTAAGAC7800.026.5123833
AGAGTAC29800.026.10191510-11
AGGTATA4400.025.6359376
CTTAGGT4450.025.3478933