FastQCFastQC Report
Sun 14 Apr 2019
SRR6576045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922275
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16190.17554417066493183No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13830.1499552736439782No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11840.1283781952237673No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11620.12599278956927162No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11310.12263153614702772No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11210.12154726084952969No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10760.11666802201078855No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9510.1031145807920631No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9500.1030061532623133No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9360.10148816784581606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.061.100811
GTATCAA16650.058.4332121
TCAACGC23550.040.104314
ATCAACG23800.039.8804743
CAACGCA24250.038.946665
AACGCAG25000.037.5903056
CTTAGGT3650.036.0454983
GTCTTAG4550.035.1213461
TATCAAC28000.034.245172
AGGTATA3800.033.3861276
TAGGTAT4000.032.8915185
ACGCAGA30250.031.0663687
TTAGGTA4250.029.8511264
GTACATG39900.029.449011
TACATGG40400.028.8534342
CGCAGAG33250.028.4047058
GTATAAA2500.028.2003771
ACATGGG41450.028.1133773
GGTATAG4550.027.882927
GCAGAGT34550.027.6064349