FastQCFastQC Report
Sun 14 Apr 2019
SRR6576045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922275
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17080.18519422081266435No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12480.13531755712775473No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11940.12946247052126536No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10920.1184028624867854No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10830.11742701471903717No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10450.11330676858854463No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10180.11037922528529993No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9660.10474099373831015No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9610.10419885608956114No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG9600.10409042855981133No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9520.10322300832181291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG156.901887E-493.9996262
GTATCAA17050.058.5189741
GGTATCA6300.050.0517731
TCAACGC23250.043.0532234
ATCAACG23750.042.1468353
CAACGCA24300.041.386295
AACGCAG24850.040.659416
TAGACCG651.4142084E-436.149785
TATCAAC28800.034.7602732
ACGCAGA30450.032.871377
GTACTAA753.24917E-431.3757381
CGCAGAG33250.030.2659138
GTACATG40150.029.53931
TACATGG41350.028.87052
GCAGAGT35000.028.618269
ACATGGG41500.028.4233073
GTCTTAG4800.026.473281
CATGGGG24100.025.9348434
TAGGTAT4050.025.5279925
AGAGTAC30950.024.99406610-11
Produced by FastQC (version 0.11.2)