FastQCFastQC Report
Sun 14 Apr 2019
SRR6576046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690009
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27460.16248434179936322No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG24270.14360870267554787No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA23100.13668566262073162No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA22380.1324253302793062No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22210.13141941847646965No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21950.12988096513095493No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21190.12538394765945032No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20630.12207035583834168No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20090.11887510658227264No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG19550.11567985732620359No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG19120.1131354921778523No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18770.1110644972896594No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA18630.11023609933438225No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18320.10840178957626852No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT18270.10810593316366954No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18050.10680416494823401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30400.061.712851
GGTATCA10100.057.261141
TCAACGC41950.044.1415674
ATCAACG41950.044.0295333
CAACGCA43750.042.540315
AACGCAG44750.041.5896876
TATCAAC51450.036.829512
ACGCAGA56700.032.90727
CGCAGAG59000.031.4650638
GTACATG78000.030.1405891
GCAGAGT63150.029.4717089
ACATGGG78250.029.1300283
TACATGG79500.029.0987452
ACCTAAG9350.026.1497781
GGTACCT11000.026.0627778
AGAGTAC56850.024.6359510-11
TAAGACA13750.023.9264814
GGGTACC12100.023.6934347
CTAAGAC13000.023.4992243
CATGGGA38550.023.2858724
Produced by FastQC (version 0.11.2)