FastQCFastQC Report
Sun 14 Apr 2019
SRR6576046_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576046_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690009
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27930.16526539207779367No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23510.1391116852040433No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT22160.13112356206387066No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21500.12721825741756407No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21410.1266857158748859No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21060.12461472098669298No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20620.1220111845558219No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20340.12035438864526757No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20180.11940764812495082No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG20080.11881593529975285No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG19840.1173958245192777No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT19250.11390471885060968No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19200.11360886243801067No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18870.1116562101148574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30950.061.398211
GGTATCA9950.056.3942951
ATCAACG40000.045.943623
TCAACGC40200.045.8319554
CAACGCA41600.044.5141835
AACGCAG43000.042.9555856
TATCAAC49650.037.3926472
GTCTTAG9300.035.4916651
CTTAGGT7500.035.0941853
ACCTAAG10850.034.7673451
ACGCAGA52750.034.6574827
TAGGTAT7300.033.479275
CGCAGAG54850.033.3305748
AGGTATA7100.033.0984156
TTAGGTA7500.031.9607754
GCAGAGT58650.031.4114449
GGTATAG7900.029.7449157
GTACATG73200.029.052051
TACATGG73800.028.277112
ACATGGG73300.027.89293
Produced by FastQC (version 0.11.2)