FastQCFastQC Report
Sun 14 Apr 2019
SRR6576047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561532
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27040.1731632781140572No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22730.14556217868093643No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21880.14011880640294275No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21140.13537987053739534No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20110.12878378412994418No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20060.12846358576065045No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19940.12769510967434544No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19830.1269906732618992No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG18370.11764088087852186No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18300.11719260316151062No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18240.11680836511835813No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17730.11354234175156193No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG17690.11328618305612692No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT17670.11315810370840942No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA17560.1124536672959632No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17390.11136499284036445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.062.4112471
GGTATCA8550.061.577681
TCAACGC39100.046.6263774
ATCAACG39150.046.5668223
CAACGCA41250.044.0822455
AACGCAG43200.042.092426
TATCAAC49750.037.2284162
ACGCAGA52000.034.9690867
CGCAGAG54500.033.278798
GCAGAGT57950.031.2164829
GTCTTAG8000.029.3799761
GTACATG71500.028.6649931
TACATGG72050.028.3156912
ACATGGG72900.028.0374413
ACCTAAG8250.027.919881
GCGTAAG700.00882967926.861691
TAGGTAT6300.026.1038275
GCTTATA1659.306859E-825.6407052
CTTAGGT6600.025.6292113
AGAGTAC53050.025.41986310-11
Produced by FastQC (version 0.11.2)