FastQCFastQC Report
Sun 14 Apr 2019
SRR6576050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354952
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16340.12059467789264859No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14940.11026220855056121No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14750.10885994485413505No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14570.10753148451015239No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14320.10568640069906535No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14240.1050959738795175No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13830.10207003642933477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18650.061.078981
GGTATCA6000.049.4247551
TCAACGC26100.042.853464
ATCAACG26700.042.0664673
CAACGCA27400.040.9917765
AACGCAG28600.039.2718436
TATCAAC34200.032.842582
ACGCAGA36800.030.5210537
CGCAGAG38300.029.4679898
GTCTTAG5400.028.765731
GTACATG49450.028.4616531
ACATGGG50450.027.386443
TACATGG51400.027.3384282
TTACGCT700.00884137526.8541474
GTATAGG3900.026.5529821
GCAGAGT43400.026.005169
AGAGTAC36100.024.35972610-11
GTTCTAG2351.9826984E-1024.0363561
AGGTATA2352.0190782E-1023.9973266
ATGGGAG15350.022.9616095
Produced by FastQC (version 0.11.2)