FastQCFastQC Report
Sun 14 Apr 2019
SRR6576051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444650
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19570.13546533762503027No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18970.13131208251133492No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18070.12508219984079189No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18000.12459765341086076No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16670.11539127124216939No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16210.11220710898833627No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15640.10826151663032568No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15470.10708476101477866No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15090.10445436610943828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24700.060.5211521
GGTATCA7400.057.807831
TCAACGC30000.048.2521064
ATCAACG30850.047.074983
CAACGCA31000.046.695595
AACGCAG32300.044.8161966
TATCAAC40300.037.093612
ACGCAGA41300.035.0499577
CGCAGAG45900.031.844518
GTACATG56250.031.8404711
TACATGG56500.031.3667812
CGTATAG1201.6501508E-731.3390451
ACATGGG57800.030.1670553
GCAGAGT49900.029.3860269
GTCTTAG6600.025.6410351
CATGGGG32850.024.8943444
TTAGGTA3100.024.2574464
AGAGTAC42950.023.63618510-11
AACGTAG1002.3019311E-823.49940392-93
GGGTACC10550.022.2743157
Produced by FastQC (version 0.11.2)