FastQCFastQC Report
Sun 14 Apr 2019
SRR6576051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444650
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17970.124389990655176No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17950.12425154881805281No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16550.11456062021943031No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15320.10604644723635484No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15230.10542345896930051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.059.8873751
GTATCAA23600.057.2734031
TCAACGC31000.043.6642574
ATCAACG31650.042.6190223
CAACGCA32200.041.5991335
AACGCAG33150.040.4070056
TATCAAC38550.034.7468762
ACGCAGA41600.032.311197
CGCAGAG44050.030.194018
GTACATG56150.029.1885531
ACATGGG56650.028.7888853
TACATGG56300.028.7174152
GCAGAGT49800.026.8021269
GTCTTAG8250.025.1178131
AGAGTAC43000.023.77229510-11
CATGGGA27150.022.331364
TAAGACA8650.021.7339764
ACCTAAG4650.021.2691141
AGTACAT38800.020.77364312-13
GGTACCT8650.020.6465638
Produced by FastQC (version 0.11.2)