FastQCFastQC Report
Sun 14 Apr 2019
SRR6576052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284580
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19130.14892026966012237No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17550.13662052966728425No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16920.13171620296127917No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16700.13000358093695996No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15690.12214108891622165No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15410.11996138815799717No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14300.11132043158075013No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14270.1110868922137975No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13920.10836226626601692No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT12880.10026623487832599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.062.022421
GGTATCA6850.058.324961
TCAACGC29300.045.384724
ATCAACG29750.044.698233
CAACGCA30700.043.315065
AACGCAG31200.042.7715076
TATCAAC38400.034.7626762
ACGCAGA40450.032.990637
CGCAGAG42800.031.0694458
ACCTAAG4350.030.2548391
GTACATG53700.028.8845581
TACATGG54050.028.2627072
GCAGAGT47700.028.1722539
ACATGGG54500.027.4170973
AGAGTAC41250.024.77474810-11
GTACATA2850.024.7384531
CTAAGAC7800.023.494213
TAAGACA8450.023.355194
GTCTTAG5700.023.0892221
GTATATA2453.3833203E-1023.0219041
Produced by FastQC (version 0.11.2)