FastQCFastQC Report
Sun 14 Apr 2019
SRR6576052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284580
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17010.13241682106213704No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16510.12852449827959334No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15970.12432078967444612No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14300.11132043158075013No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14280.11116473866944838No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14150.11015273474598701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22100.059.2020341
GGTATCA7300.053.510541
TCAACGC28200.045.494074
ATCAACG28650.044.615473
CAACGCA29350.043.871625
AACGCAG30350.042.4244426
TATCAAC35950.035.5572472
TAGGTAT3200.035.24545
CTTAGGT3200.035.24543
ACGCAGA37500.034.2102057
GTCTTAG5900.033.5028231
TTAGGTA3250.033.25724
CGCAGAG41050.031.5015078
ACCTAAG3750.030.1206281
GTACATG51250.028.8349931
AGGTATA3950.028.5521246
TACATGG51900.028.16122
ACATGGG52350.027.73853
GCAGAGT46800.027.7316069
TTAGACG700.00884202426.853644
Produced by FastQC (version 0.11.2)