FastQCFastQC Report
Sun 14 Apr 2019
SRR6576053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962444
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21610.22453254423114485No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18090.18795898774370248No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15420.16021711393078455No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14220.14774885603733828No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13600.14130692279239104No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12620.13112451217940993No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12310.1279035455569363No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12240.12717623051315194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.055.9085541
ATCAACG29600.042.0774463
CAACGCA29800.041.9527665
AACGCAG32900.039.428336
CGTAAAT757.442648E-637.5997733
TCAACGC33350.037.34614
TATCAAC34500.037.331192
GGTATCA8750.035.9920921
ACGCAGA35900.034.6933827
CGCAGAG36550.034.333588
TGATCGC2950.033.4574249
GCAGAGT39700.031.609389
GTACTAG4500.030.2918511
GTACATG48150.030.1649441
TAGGTAT5350.029.8689775
TAGGCAT4750.029.684035
AGGTATA5250.029.5426796
TACATGG49100.029.3898412
ACATGGG48650.029.2721773
CTTAGGT5350.028.9904773
Produced by FastQC (version 0.11.2)