FastQCFastQC Report
Sun 14 Apr 2019
SRR6576053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962444
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22750.23637738922991886No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16800.17455561050824775No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14720.15294396349294087No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13560.14089131419594284No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13010.13517669599477997No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11200.1163704070054985No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9810.10192800827892325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23650.056.3908461
ATCAACG31500.042.074313
CAACGCA32000.041.2700275
GTACTAG4250.037.7001651
GGTATCA8900.037.594271
AACGCAG36400.037.0560346
TATCAAC36250.036.6907732
ACTATCG906.1910396E-736.553923
TCAACGC36200.036.3519674
CGCAGAG39100.033.6557858
ACGCAGA39550.033.3916787
GCAGAGT41750.031.5195489
GTACATG50050.030.035851
TACATGG50850.029.6682872
ACATGGG50250.029.2743113
ACCTAAG9250.029.0393181
TAGGCAT5850.027.3150145
GGTAATC2253.6379788E-1227.154348
TAAGACA11450.026.2695664
AGAGTAC40000.026.20132610-11
Produced by FastQC (version 0.11.2)