FastQCFastQC Report
Sun 14 Apr 2019
SRR6576055_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576055_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169829
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16440.140533360003898No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16100.1376269523152529No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14240.1217271926067827No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12720.10873384058695758No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12620.1078790147961796No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12140.10377585100044535No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11760.1005275129954891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20950.060.2556041
GGTATCA7050.054.786231
ATCAACG28350.045.091653
TCAACGC28850.044.4730724
CAACGCA30050.042.071515
AACGCAG30700.041.1807446
TATCAAC33900.037.570752
ACGCAGA38200.033.0941057
CGCAGAG40300.031.36968
GTCTTAG6150.030.6359631
GCAGAGT43800.029.077499
GTACATG48600.027.815851
TACATGG49900.027.219342
ACATGGG51000.026.447953
TAGGTAT5200.025.3066675
CTTAGGT5050.025.1276973
AGGTATA4900.024.9377676
TTAGGTA4950.024.685874
AGAGTAC38100.024.11479210-11
GTATAGG3400.023.5513971
Produced by FastQC (version 0.11.2)