FastQCFastQC Report
Sun 14 Apr 2019
SRR6576056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036450
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15030.14501423127020116No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14580.14067248781899755No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13730.13247141685561292No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12850.12398089632881469No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12760.12311254763857397No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12170.11742003955810701No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11430.11028028366057215No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10950.10564909064595493No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10930.1054561242703459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18050.061.453731
GGTATCA6300.057.4465071
TCAACGC23800.045.998724
ATCAACG24450.044.775853
CAACGCA25200.043.6296845
AACGCAG25950.042.3687136
TATCAAC31650.034.7500652
ACGCAGA32750.033.7150157
GTACATG44400.032.0754781
CGCAGAG34850.031.5485848
TACATGG46200.031.1309912
ACATGGG46300.029.7340053
GTCTTAG6700.028.7622261
GCAGAGT39250.028.1316389
TTACGCA700.00884864326.8490494
GGGTACC8500.025.9803927
CTTAGGT5450.025.8637523
GGTACCT8450.025.0220328
AGAGTAC33800.024.95252610-11
TAGGTAT5300.024.8226975
Produced by FastQC (version 0.11.2)