FastQCFastQC Report
Sun 14 Apr 2019
SRR6576056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036450
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15000.1447247817067876No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13890.13401514786048532No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13320.12851560615562738No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11570.11163104828983549No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11330.1093154517825269No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11060.10671040571180472No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11010.10622798977278208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17700.059.82251
GGTATCA6600.057.7559051
TCAACGC26000.040.8471154
ATCAACG25800.040.7994773
CAACGCA27050.039.261555
AACGCAG27700.038.6795466
TATCAAC31150.033.795452
CTAGACG702.18697E-433.56593
ACGCAGA34400.031.0094267
CGCAGAG36150.029.6597358
GTACATG43350.028.0082441
TACATGG44550.027.3224872
ACATGGG44950.027.0767423
GCAGAGT39700.026.8890869
GTCTTAG6700.025.2861651
TAAGACA8450.025.0254614
AGAGTAC34650.024.97211310-11
TAGGTAT5100.024.8782545
TTAGGTA5300.024.8260974
CTAAGAC8150.024.793463
Produced by FastQC (version 0.11.2)