FastQCFastQC Report
Sun 14 Apr 2019
SRR6576057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773174
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14310.18508123656511988No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13500.17460494015577346No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12830.1659393616443388No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12130.15688577215478017No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11190.14472809484023No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA10350.13386378745275965No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9970.12894898172985642No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9650.12481019796320104No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9470.1224821320944574No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8840.11433390155385463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.11291119463406685No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8730.11291119463406685No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8640.11174716169969501No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT8450.10928975883824339No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT8250.1067030189840838No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7760.10036550634139275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23150.068.032571
GGTATCA5700.062.6847341
TCTAACG602.548768E-854.8491482
TCAACGC31700.049.815294
ATCAACG31850.049.728243
CAACGCA32400.049.4643145
AACGCAG33400.048.2647746
TATCAAC34400.045.646882
GTACTAG952.1100277E-839.5903631
CGCAGAG40600.039.4739848
ACGCAGA40750.039.4440167
GCAGAGT43150.037.250149
CCCTATA1055.095535E-835.819852
CTAACGC959.44794E-734.6303633
GTACATG52200.030.5317921
ACATGGG53100.029.7390693
TACATGG53600.029.7343182
TATAGAC1602.1318556E-929.373973
ACCTAAG5950.029.235321
ATGGGAG11250.028.4078925
Produced by FastQC (version 0.11.2)