FastQCFastQC Report
Sun 14 Apr 2019
SRR6576058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704967
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12850.18227803570947293No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12230.17348329779975516No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11470.16270265132977854No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10810.15334051097427256No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9690.13745324249220175No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9410.13348142537168406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.1322047698686605No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9160.12993516008550757No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8760.12426113562762511No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8150.11560824832935442No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8090.11475714466067206No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7710.10936682142568377No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7650.10851571775700138No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT7530.10681351041963666No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7510.10652980919674253No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7500.10638795858529548No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7310.10369279696780132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21650.063.1737371
GGTATCA7000.061.100511
ATCAACG30150.044.571473
TCAACGC30600.044.5302164
CAACGCA30250.044.4241265
AACGCAG31800.042.4065556
TATCAAC32950.041.0807652
TTATACC1003.331843E-837.5896454
GTACTAG1401.2732926E-1136.928881
CGCAGAG37000.036.4467168
ACGCAGA36800.036.389437
CTTAGGT2550.035.0099643
GCAGAGT39250.034.4771279
TATCGGC550.002716188834.1821022
TAGGTAT2900.032.4048655
AGGTATA2800.031.8840736
ACCTAAG5300.031.0379921
GTACATG46800.030.2288841
TCTTAGG4050.030.173092
ACATGGG46100.029.7618683
Produced by FastQC (version 0.11.2)