FastQCFastQC Report
Sun 14 Apr 2019
SRR6576059_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576059_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307060
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8250.26867713150524325No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5190.16902234091057122No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5160.16804533315964307No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4810.15664690939881457No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4520.14720250113984237No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4490.14622549338891422No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4020.13091903862437307No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3750.12212596886601967No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3720.1211489611150915No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.12017195336416335No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3540.11528691460952258No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3440.11203022210642873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.10975053735426302No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3270.10649384485116917No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3220.10486549859962221No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3150.10258581384745653No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3140.10226014459714713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT250.005232970756.3984384
GTACTAG601.5730329E-647.1214681
ACTAGGC708.5830834E-846.99873
TACTAGG751.4765646E-743.865452
ACCTGTA555.274882E-542.726092
GTATCAA10700.036.5521661
CGTCTAT550.002679783234.270161
AACGCAG12500.031.5831246
CTTAGGT753.2705284E-431.3324643
CAACGCA13200.029.9082625
TTAGGTA953.7441325E-529.6833884
TAGGTAT804.7735948E-429.3741865
TCAACGC13300.029.3300134
GTCTTAG1303.244022E-728.9978261
ATAGTAC650.006148075728.9222743
ATCAACG13550.028.788873
ACATGGG18200.028.4058043
GTACATG18350.028.2472021
TACATGG18750.028.0738872
GTAGGAC856.805738E-427.6462923
Produced by FastQC (version 0.11.2)