FastQCFastQC Report
Sun 14 Apr 2019
SRR6576060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901137
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17560.194864931747337No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14990.1663454058594864No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14430.16013103445979912No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13390.14859005900323702No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12980.14404025137132312No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11940.13249927591476102No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11930.13238830499690946No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11750.13039082847558142No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11030.12240092239026919No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10420.1156316964013241No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10370.1150768418120663No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10240.11363421987999604No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9990.11085994693370707No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9790.10864052857667592No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9770.1084185867409728No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9710.10775276123386344No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9020.1000957679021059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21450.056.9895481
GGTATCA8450.045.0689541
ATCAACG27100.043.8764653
CAACGCA27050.043.7838255
AACGCAG32200.038.0947466
TCAACGC32350.037.0464254
TATCAAC33800.036.722852
ACGCAGA33750.035.231187
CGCAGAG34650.034.5873538
GCAGAGT37650.031.706569
CGTTAAC1859.094947E-1230.4971071
GTATAAG4100.029.8152641
TATACTC804.7811973E-429.3738215
GTACATA650.006143009328.9331531
GTACTAG5200.028.9331531
GTACATG39600.027.426221
GTCTAGA1205.8255446E-627.426221
TAGTACT6350.027.3847274
TACATGG39650.027.2730582
GTACAGA3450.027.2558691
Produced by FastQC (version 0.11.2)