FastQCFastQC Report
Sun 14 Apr 2019
SRR6576060_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576060_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901137
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC18160.2015231868184305No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15270.16945259155933004No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14900.16534666759882238No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12380.1373819963002296No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11890.13194442132550321No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11360.12606296267937062No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11200.12428742799374569No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10750.11929373669042555No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10300.11430004538710539No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10270.11396713263355074No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10270.11396713263355074No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10030.11130383060511333No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10020.11119285968726177No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9730.10797470306956657No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9600.10653208113749629No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9590.10642111021964473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.10608819746609006No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9290.10309198268409797No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9200.10209324442343394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.059.1813161
ATCAACG27500.044.7829633
CAACGCA27900.044.3093955
GGTATCA7250.043.56971
AACGCAG31150.040.139126
TCAACGC32500.038.037914
ACGCAGA33250.037.173727
TATCAAC34800.037.1447752
CGCAGAG34050.036.3003358
GCAGAGT36750.033.7612539
GTACTAG4800.031.4308761
GTTATAC600.004158333431.336683
ATTGCGT1303.32715E-728.9261686
AGAGTAC34400.028.07536310-11
TAGGTAT1555.075708E-827.2932425
TCTTAGG1904.474714E-1027.2134342
GTACATG37100.026.4324321
TACATGG37950.025.7629622
ACATGGG38150.025.7511143
TAGGCAT5950.025.280015
Produced by FastQC (version 0.11.2)