FastQCFastQC Report
Sun 14 Apr 2019
SRR6576061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836114
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16220.19399268520799795No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15280.18275019913552457No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14380.17198611672570965No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12340.14758752993012914No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11610.1388566630866126No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11480.13730185118297267No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11020.1318002090624006No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10900.13036499807442525No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9950.11900291108628727No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9810.11732849826698273No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9770.11685009460432429No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9760.11673049368865968No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9560.11433847537536748No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9230.11039164515843534No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9130.10919563600178923No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8920.10668401677283242No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8800.10524880578485708No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC8720.1042919984595402No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8610.1029763883872295No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8560.10237838380890645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.053.8048821
GGTATCA8750.048.8781621
ATCAACG26450.043.1676333
CAACGCA26500.043.0861855
AACGCAG31650.037.5599026
TCAACGC31550.036.1896634
ACGCAGA32400.035.675317
GAACCGT1452.0008883E-1135.6453066
TATCAAC33800.034.4839132
CGCAGAG33650.034.3500758
GCAGAGT35500.032.4276439
CGTTAAC1401.8948413E-830.2059259
TGGGTAC4850.030.0329236
GTACTAG5450.028.4576471
ATGGGTA5150.028.2834325
GGGTACC5000.028.1921947
TAGTACT5750.027.7836154
GTACTGT6450.027.6822596
GTACATG34600.027.3024431
TACATGG35000.027.2589762
Produced by FastQC (version 0.11.2)