FastQCFastQC Report
Sun 14 Apr 2019
SRR6576062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497082
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18970.12671316601228255No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17380.11609250528695156No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15900.1062066072533101No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15410.10293357344487476No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15280.10206521753651436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28650.058.583161
GGTATCA9650.057.0016861
TCAACGC36900.044.4511684
ATCAACG36950.044.391023
CAACGCA38250.042.88235
AACGCAG39300.041.8561866
TATCAAC45400.036.658662
ACGCAGA49450.033.169837
CGCAGAG53100.030.9783048
GTACATG64000.030.4123211
TACATGG65800.029.2231222
GCAGAGT57650.028.8594539
ACATGGG66500.028.552483
CTAAGAC11100.024.5577813
CATGGGG38050.024.2094844
AGAGTAC50150.024.17869810-11
GTCTTAG8600.024.0537831
ACCTAAG7500.023.193681
CATGGGA26350.023.008764
TAAGACA12150.022.0486854
Produced by FastQC (version 0.11.2)