FastQCFastQC Report
Sun 14 Apr 2019
SRR6576062_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576062_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497082
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17860.11929874248705148No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17040.11382142060354743No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16310.10894526819506213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28750.060.6406061
GGTATCA9650.055.6643561
TCAACGC38450.044.49844
ATCAACG38250.044.4867863
CAACGCA39300.043.535975
AACGCAG40500.042.8263176
TATCAAC44350.038.473962
GTCTTAG9000.034.0306741
ACGCAGA51150.033.3535737
TAGGTAT6200.031.8417535
CGCAGAG53900.031.5646638
GTACATG67150.030.2434221
CTTAGGT6850.030.1936763
TACATGG68700.029.5583972
GCAGAGT58300.029.1018099
ACATGGG69350.029.078013
TTAGGTA6800.029.0321854
GGTATAG6700.028.7601017
AGGTATA6650.028.273386
TCTTAGG9850.025.2926042
Produced by FastQC (version 0.11.2)