FastQCFastQC Report
Sun 14 Apr 2019
SRR6576063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325163
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17820.13447402319563706No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16070.12126810060347293No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14930.11266538531486317No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13620.10277980897444314No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13470.10164787275225765No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13340.10066686135969688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.059.0051651
GGTATCA7200.057.4408951
TCAACGC29700.044.9298064
ATCAACG29600.044.922863
CAACGCA30850.043.1026465
AACGCAG32000.041.5536466
TATCAAC36500.036.825122
ACGCAGA40050.033.2014167
CGCAGAG43650.030.4631548
TACATGG53700.029.6685582
GTACATG54800.029.2445411
GCAGAGT47400.028.2513479
ACATGGG56150.028.032883
AGAGTAC40950.024.55458310-11
CTTAGGT5550.024.551483
TTAGGTA5800.024.3033394
TAGGTAT5050.024.1910465
GTCTTAG8600.024.0450251
ATTATCC7900.023.7906113
ACCTAAG6800.022.8074131
Produced by FastQC (version 0.11.2)