FastQCFastQC Report
Sun 14 Apr 2019
SRR6576063_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576063_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325163
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16600.12526760858852837No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16540.12481483409965415No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14890.11236353565561369No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14010.10572284315212543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.065.6695561
GTATCAA24250.060.524311
ATCAACG32450.044.7478033
TCAACGC32650.044.329774
CAACGCA33400.043.3343435
AACGCAG34850.041.5313346
TATCAAC38400.038.0618482
ACGCAGA42950.033.2612347
CTAGACG902.5970761E-531.328293
CGCAGAG47250.030.3543578
GTACATG60900.030.2799931
ACATGGG61000.030.0443443
TACATGG62000.029.6377892
GCAGAGT50600.028.623539
TGTAACG700.00884018526.8548472
GTACCGT1406.378523E-726.8528216
ACCTAAG7550.025.5460591
TAAGACA9850.025.285274
CTAAGAC9150.025.1653463
AGAGTAC45200.024.18842110-11
Produced by FastQC (version 0.11.2)