FastQCFastQC Report
Sun 14 Apr 2019
SRR6576064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037482
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17600.1696414973946536No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15150.14602662985960238No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13460.12973719062113848No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13130.12655641254498873No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10800.10409819158308288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.063.7256971
GTATCAA22100.058.4911161
TCAACGC28850.044.6366354
ATCAACG28950.044.4824523
CAACGCA30400.042.3607565
AACGCAG31400.041.3110436
TATCAAC34200.037.9343382
GTCTTAG7900.033.320421
ACGCAGA38900.033.225377
TAGGTAT6250.031.5831895
CGCAGAG41450.031.1813478
CTTAGGT6250.030.831213
GCAGAGT43250.029.8836299
TTAGGTA6150.029.8041154
AGGTATA6150.029.8041156
GAGTACG804.7812832E-429.3742479
GTACATG46550.029.2838271
GGTATAG6400.028.639897
ACATGGG47400.028.1596153
TACATGG47750.028.0556932
Produced by FastQC (version 0.11.2)