FastQCFastQC Report
Sun 14 Apr 2019
SRR6576065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919608
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15180.16507033431636087No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14830.16126436481631304No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12840.13962470965889814No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12740.1385372898017416No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9310.10123878870127272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19100.059.8011361
GGTATCA6450.051.741041
TCAACGC24350.046.6968574
ATCAACG24450.046.3136983
CAACGCA25450.044.6785245
AACGCAG26200.043.5788966
TATCAAC29600.038.27032
ACGCAGA31600.035.9831777
CGCAGAG32850.034.470928
GCAGAGT36200.031.5405259
GTCTTAG6000.031.3361261
GTACATG39350.030.8185021
TACATGG40000.030.4352112
ACATGGG39700.029.7067053
GGTATAG3700.029.2076997
AGGTATA3800.028.4390756
AGAGTAC32700.025.5766410-11
TAGGTAT4450.025.3409255
TAGTACC1301.0899812E-525.3003144
ACCTAAG6250.024.818211
Produced by FastQC (version 0.11.2)