FastQCFastQC Report
Sun 14 Apr 2019
SRR6576068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139396
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17720.15552099533437014No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16550.14525239688396308No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13100.11497319632507048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18200.060.1786771
GGTATCA6300.052.2294651
TCAACGC25850.041.4530144
ATCAACG26450.040.3353
CAACGCA26950.040.1098375
AACGCAG28050.038.8720056
TATCAAC32250.034.1070562
TAGGTAT4550.032.0207985
ACGCAGA34700.031.287037
GTACATG46050.030.5210631
CGCAGAG36150.030.0320858
GTATACG804.776978E-429.3790721
TACATGG47450.029.1252172
ACATGGG46700.028.9839423
AGGTATA4400.028.8398466
GTCTTAG8000.028.7914921
CTTAGGT4650.028.300033
CGGGCGT700.0088375826.8561526
GCAGAGT41150.026.3829889
ACCTAAG7200.025.4618641
Produced by FastQC (version 0.11.2)