FastQCFastQC Report
Sun 14 Apr 2019
SRR6576068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139396
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18580.16306885402441293No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16350.14349708090953453No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12840.11269128555831336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.062.194971
GGTATCA4900.059.639321
TCAACGC23850.043.5569534
ATCAACG23900.043.0724753
CAACGCA24550.042.3150065
AACGCAG25200.041.4100766
TATCAAC29150.035.4762542
GTACTAG851.7208353E-533.271271
ACGCAGA33000.031.759117
GTACATG42650.031.1650031
CGCAGAG33900.030.5000368
TACATGG44000.029.6992662
ACATGGG44550.028.8050443
GCAGAGT38250.027.1542769
GTCTTAG6400.026.5130461
TAGCGCA909.520245E-426.109889
ATAGCGC909.520245E-426.109888
ACCGTTC1301.0882148E-525.3064988
AGAGTAC33000.024.92306910-11
ACCTAAG8350.024.8372381
Produced by FastQC (version 0.11.2)